From ELISA To Western Blot: The Various Aspects Of Antibodies In Laboratories

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Antibodies serve as one of the most important additions to biomedical research. These are adept at binding with certain molecules, otherwise known as antigens. This helps the researcher in targeting and detecting certain proteins, cells, and other molecules that are a part of their work in understanding biological samples. Reputed antibodies suppliers provide biomedical research laboratories with antibodies in bulk for various purposes. Read further in this article on the various aspects, features, and uses of antibodies in biomedical research.

Examining The Aspects Of Antibodies In Labs

Antibodies, for the longest time, have served as invaluable tools in biomedical research laboratories. Their specificity, versatility, and ability can help researchers recognize as well as target certain molecules. The elaborate research and observation of biological processes, disease diagnosis, and therapeutic development fuels better knowledge in the researcher. Diseases and biological systems are better understood with the help of antibodies. Here are the various aspects to understand about antibodies.

ELISA And Antibodies

ELISA is a very popular antibody-based technique used widely in the field of biomedicine. The proteins or antigens present in a certain biological sample can be easily detected with the help of this. It is heavily reliant on the binding affinity between antibodies and their corresponding targets.

Western Blotting

Alternatively known as immunoblotting, western blotting is a technique that uses antibodies to identify and categorize proteins. These are separated with the help of gel electrophoresis, then transferred to a membrane, and finally probed with antibodies.

The Immunochemistry Behind It All

Immunochemistry is the science of interaction between antibodies and antigens. Antibodies can locate certain areas on antigens that are called “epitopes”. The bond between an antibody and an epitope is highly specific, close to that of a lock-and-key. Antibodies’ exhibit of affinity for their targets helps in much more accurate research.

Antibody Selection For Labs

Not just any; the right antibodies are important for a successful laboratory experiment or research. Considering specificity, cross-reactivity, and validation is a crucial step if you wish to achieve good results. Validated antibodies need to undergo rigorous testing procedures to be termed useful for laboratory research.

Antibody Conjugation

Antibodies can be labeled with the help of enzymes, fluorophores, or nanoparticles. This helps in proper visualization and further quantification of the desired results. Flow cytometry and immunohistory especially benefit from this.


Non-specific binding remains a prominent issue in this case. Proper antibody concentrations, optimizing blocking reagents, and verifying the quality of antibodies are all common troubleshooting methods.

Advanced Applications

With their impressive lab performance, antibodies have grown to have even more advanced applications in biomedical research laboratories. A good example of this is flow cytometry, which uses fluorescently labeled antibodies.


Antibodies are one of the most important components of biomedical research. Its diverse applications in this field help researchers find newer ways to cure illnesses and also make possible developments in the field. Looking to find a trusted supplier for your laboratory needs? Get premium quality primary and secondary antibody kits from Universal Technology, a leading name to trust when you require crucial biomedical research apparatus.

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