This comes as UK inflation hit a 40-year high.
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The NI Business Brexit Working Group said the two sides should redouble their efforts to end the impasse.
The group said the EU needed to show more ambition on the issues of controls on food products and parcels going from Great Britain to Northern Ireland.
It also warned that part of the UK's plan to override the protocol would "create a myriad of reputational, legal and commercial risks".
The protocol is the post-Brexit trading arrangement that was agreed by the UK government and the EU in 2019.
It keeps Northern Ireland inside the EU's single market for goods, preventing checks on goods at the Irish border and giving Northern Ireland manufacturers better access to the EU than companies in other parts of the UK.
It also means there are checks and controls on goods entering Northern Ireland from Great Britain, leading to added cost and complexity for importing businesses.
The UK government said the protocol was not working.
It is planning to override most of the deal if the EU does not agree to radical changes.
Both sides said they wanted a negotiated settlement but there have been no high level political talks since February.
The working group, whose members include the CBI, NI Chamber of Commerce and Ulster Farmers' Union, said "for too long this issue has been dominated by inflexibility and intransigence but Northern Ireland is now facing into the most difficult of winters".
The EU made proposals last year to simplify the paperwork needed to move goods from Great Britain to Northern Ireland.